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KMID : 0984720040360020059
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Infection and Chemotherapy
2004 Volume.36 No. 2 p.59 ~ p.67
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Characterization of Korean Leptospira interrogans Isolates by Pulsed-Field Gel Electrophoresis of Not I Digests of DNA
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Yim Eun-Kyoung
Chang In-Ae
Lee Jin-Sang
Kim Yoon-Won
Woo Soo-Dong
Ahn Jee-Yin
Park Seung-Kee
Cho Min-Kee
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Abstract
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Background : Many strains of Leptospim interro-gues have been isolated in Korea since 1984. Most isolates were identified as serovar lai by serological methods. The pulsed field gel electrophoresis (PFGE) patterns of Korean isolates have not been investigated currently.
Methods : 29 reference strains and 29 Korean isolates of Leptospira interrogans were characterized by PFGE. Chromosomes were digested by the Not I restriction enzyme and subsequently PFGE was performed in CHEF-DRII (Bio Rad Lab) with 3 pulse times (30 seconds 13 hours, 60 seconds 13 hours, 120 seconds 14 hours) at 150 V (4.5 V/cm).
Results : 12 serogroup reference strains and most 17 serovars reference strains in the serogroup Icterohaernoffhagie showed the unique Not I restriction patterns. Most isolates identified serologically as serovar lai showed the same PFGE patterns as the serovar lai reference strain. The strain HM3 and 18R identified serologically as new serovars veonchon and hongchon respectively showed the same PFGE patterns as serovar lai. The strain AP31, CH88-19 and NR13 that were different fi-om scrovar- lai by serological methods showed the PF(:E patterns indistinguishable from serovar lai reference strain. The strain I-112 that was identified as serovar lai. and the strain -OlM that was different from serovar lai serologically showed the PF(tE patterns slightly- different from serovar lai reference strain.
Conclusion : The PFGE profile of most Korean iso kites IAptosJ)iri interrogans serologically identified as scrovar lei is identical to the reference strain serovar Iai. I FGF. analysis thus may be applied to identify scrovar of isolates and to investigate the genetic diversity of related scrovar.
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KEYWORD
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PFGE, Icterohaemorrhagiae, Leptospira interrogans, Not I
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